实验室按照“学科方向与国家目标、原始创新与产业发展相结合”的方针，围绕“海洋产物资源与生物转化工程”主题，以绿色环保、节能减排为导向，以海洋天然产物资源的可持续利用和开发有用的海洋活性功能产品为目标，突破海洋生物酶催化与转化的关键技术，完成新型海洋生物酶制剂的开发及其在生物催化转化中的应用，实现生物技术路线替代传统化学工艺的目标，完善酶催化机制－制备工艺－催化转化过程-产品开发的高通量、高效率生物催化产品的产业化技术体系，开发新一代的绿色制造技术。获得一批具有国际先进水平的成果和重大经济效益或社会效益的知识产权，通过现代生物技术开发出能应用于工业、农业、环保和提高人民生活质量的高技术产品。

研究室的主要研究方向包括：海洋微生物资源保藏与利用、海洋生物酶制品与功效研究、海洋活性蛋白结构功能。具体研究内容包括：海洋微生物资源收集、保藏及利用；海洋微生物代谢调控和产物的高效表达；海洋活性蛋白质结构功能和分子进化；海洋生物酶催化与转化技术；海洋产物开发的工程化技术及系统集成和应用技术等。

近年来，实验室承担了国家重点研发计划、国家863项目、科技部国际合作专项、发改委专项、国家自然科学基金、山东省重大专项等课题180余项；获得山东省科技进步一等奖、中国机械工业科学技术奖二等奖等18项科技奖励；在国内和国际学术刊物上发表文章380余篇，其中SCI收录180余篇。申请发明专利120余项，授权发明专利80余项。

成果展示：专利

发表文章

1.A Catalytic Binding Site Together with a Distal Tyr in Myoglobin Affords Catalytic Efficiencies Similar to Natural Peroxidases.Ping Zhang, Hong Yuan, Jiakun Xu, Xiao-Juan Wang, Shu-Qin Gao, Xiangshi Tan, and Ying-Wu Lin*.ACS Catalysis.2020,10(1):891-896

2.“Aptamer-locker” DNA coupling with CRISPR/Cas12a-guided biosensing for high-efficiency melamine analysis.Bin Qiao*, Jiakun Xu*, Wenhao Yin, Wanmeng Xin, Lixin Ma, Jie Qiao*, Yi Liu*.Biosensors and Bioelectronics, 2021, 183, 113233:1-7

3.Laminaria japonica hydrolysate promotes fucoxanthin accumulation in Phaeodactylum tricornutum.Zhi-Peng Wang, Pei-Kang Wang, Yan Ma, Jia-Xue Lin, Cheng-Long Wang,Yu-Xiang Zhao, Xin-Yue Zhang, Bei-Chen Huang, Shou-Geng Zhao, Lei Gao, Jing Jiang,Hai-Ying Wang, Wei Chen*.Bioresource Technology,2022,344(126117):1:4

4.Production, Biosynthesis, and Commercial Applications of Fatty Acids From Oleaginous Fungi.Xin-Yue Zhang, Bing Li , Bei-Chen Huang, Feng-Biao Wang, Yue-Qi Zhang, Shao-Geng Zhao, Min Li, Hai-Ying Wang, Xin-Jun Yu*, Xiao-Yan Liu, Jing Jiang* and Zhi-Peng Wang*.Frontiers in Nutrition.2022,9,873657:1-15

5.Cloning and characterization of fructose-1,6-bisphosphate aldolase from Euphausia superba.Jikun Xia, Wanmeng Xin, Fang Wang, Wancui Xie, Yi Liu* and Jiakun Xu*.Int. J. Mol. Sci. 2022, 23, 10478:1-15

6.Design and Engineering of an Efficient Peroxidase Using Myoglobin for Dye Decolorization and Lignin Bioconversion.Wen-Jie Guo, Jia-Kun Xu, Sheng-Tao Wu, Shu-Qin Gao, Ge-Bo Wen, Xiangshi Tan and Ying-Wu Lin*.Int. J. Mol. Sci.2022, 23,413:1-15

7.Effects of Pore Size and Crosslinking Methods on the Immobilization of Myoglobin in SBA-15.Hengmin Miao, Maosheng Li, Xiaochun Sun, Jikun Xia, Yanqing Li, Jiao Li*,Fang Wang* and Jiakun Xu*.Front. Bioeng. Biotechnol.2022,9,827552:1-8

8.Surface functionalization of SBA-15 for immobilization of myoglobin.Hengmin Miao, Maosheng Li , Fang Wang, Jiao Li*, Ying-Wu Lin* and Jiakun Xu*.Front. Bioeng. Biotechnol.2022,9,827552:1-9

9.Spiro-oxindole skeleton compounds are efficient inhibitors for indoleamine 2,3-dioxygenase 1: An attractive target for tumor immunotherapy.Yan, Daojing, Xu, Jiakun, Wang, Xiang, Zhang, Jiaxing, Zhao, Gang, Lin, Yingwu* and Tan, Xiangshi*.Int. J. Mol. Sci.2022,23,4668:1-14

10.Application of engineered heme enzymes based on myoglobin with high peroxidase activity for efficient degradation of various emerging pollutants.Guang-Rong Wu, Jia-Kun Xu, Li-Juan Sun, Ying-Fu Li, Shu-Qin Gao, Ying-Wu Lin,.Journal of Environmental Chemical Engineering,2023,11,111471:1-9

11.Construction of a myoglobin scaffold-based biocatalyst for the biodegradation of sulfadiazine and sulfathiazole.Weikang Zhang,Yingwu Lin,Xiangmin Meng,Qiaoning Wang,Lingxin Chen*,Jiakun Xu*.Journal of Hazardous Materials,2024,465,133147:1-10

12.Comparative Study on Enzymatic Characteristics of Two κ-Carrageenases from Carrageenan-degrading Bacterium Catenovulum agarivorans DS2.Chengcheng Jiang, Wei Wang, Jingjing Sun, Jianhua Hao, Xiangzhao Mao.Journal of Agricultural and Food Chemistry,2024,72,22,12665–12672

13.Systematic review on carrageenolytic enzymes: from metabolic pathways to applications in biotechnology.Chengcheng Jiang, Yuqi Ma, Wei Wang, Jingjing Sun, Jianhua Hao*,Xiangzhao Mao.Biotechnology Advances.2024,73,108351:1-21

14.Biochemical Characterization of a Heat-Resistant κ-Carrageenase Capable of Tolerating High Temperatures up to 100 ℃.Chengcheng Jiang, Wei Wang, Jingjing Sun, Jianhua Hao,* and Xiangzhao Mao.J. Agric. Food Chem. 2024, 72, 24, 13824–13833

15.Simultaneous One-Step Preparation of β/κ-Carrapentaose and 3,6-Anhydro-D-galactose by Cascading κ-Carrageenase and an Exo-α-3,6-Anhydro-D-galactosidase.Chengcheng Jiang*,Wei Wang,Jingjing Sun,Jianhua Hao,Xiangzhao Mao*.Journal of Agricultural and Food Chemistry,2024,72,47,26274–26282

16.Engineering the Non-catalytic Domain to Enhance Catalytic Activity and Thermal Stability of a Nκ2-Producing κ-Carrageenase.Chengcheng Jiang*,Xiangzhao Mao*.Journal of Agricultural and Food Chemistry,2024,72,47,26348–26355

17.Construction of biocatalysts based on P450BM3 for the degradation of non-steroidal anti-inflammatory drugs.Yadan Yang, Weikang Zhang, Fang Wang, Dong Li, Xiangmin Meng, Xiaochun Sun, Jiakun Xu*.Journal of Hazardous Materials,2024,480,136097:1-11

18.Purification, immobilization, evolution, and characterization of D-allulose 3-epimerase from Antarctic Microbacterium.Jingqi Li, Jingjing Sun, Wei Wang, Chengcheng Jiang, Jianhua Hao*.International Journal of Biological Macromolecules.2025,310,143294:1-12

19.Rational Design of Alginate Lyase AlgL3199 for Thermal Stability and Specific Activity Enhancement.Zhifang Chen, Jianhua Hao, Yingying Guo, Tiantian Zhu, Jingjing Sun, Wei Wang, Chengcheng Jiang, Haiying Wang*.Journal of Agricultural and Food Chemistry.2025,73,24,15245–15258

20.Regioselective aromatic O-demethylation with an artificial P450BM3/sugar alcohol oxidase peroxygenase system.Sijia Zhao, Yingwu Lin, Jinghan Wang, Dong Li, Fang Wang, Osami Shoji, Jiakun Xu* .International Journal of Biological Macromolecules.2025,309,142768:1-8

21.Construction of P450 scaffold biocatalysts for the biodegradation of five chloroanilines.Ridong Yang, Osami Shoji, Yingwu Lin, Fang Wang, Hongxia Che, Jiakun Xu*.Journal of Hazardous Materials,2025,488,137305:1-11

22.Combined Acid and Enzymatic Hydrolysis Using an Endolytic κ-Carrageenase RsKC16A for Efficient Preparation of Carrageenanoligosaccharides.Chengcheng Jiang*,Xiangzhao Mao*.Journal of Agricultural and Food Chemistry.2025,73,41,26325–26333

23.Cloning, Expression and Characterization of Glutathione S-Transferase from Euphausia superba .Mengyao Chen, Jia Song, Fang Wang, Jiahao Tan, Huaying Zou, Jiakun Xu*.Journal of Agricultural and Food Chemistry.2025,73,47,30183-30195

24.Cloning, expression and characterization of xylose isomerase from Euphausia superba .Jiahao Tan, Huaying Zou, Jun Sheng, Dong Li, Fang Wang, Jiakun Xu*.International Journal of Biological Macromolecules.2025,322,146782:1-11

25.Domain-Function Analysis of the Endolytic Ulvan Lyase PcPL40A: Elucidating the Impact on Enzymatic Properties.Chengcheng Jiang, Chenghao Hu, Jianhua Hao*, Wei Wang, Jingjing Sun,  Xiangzhao Mao*.Journal of Agricultural and Food Chemistry.2026,74,16,13086–13099

26.Selective Aqueous Biocatalysis of D-Panthenol via Engineered Pantothenate Synthetase.Chaofan Wang, Wei Wang, Jingjing Sun, Chengcheng Jiang, Rui Wang, Jianhua Hao.Journal of Agricultural and Food Chemistry.2026,74,13,11015–11028

27.Enzymatic catalysis as a sustainable approach toward environmental remediation of sulfonamides: Advances, mechanisms, challenges and future perspectives.Weikang Zhang, Jiakun Xu* & Jieming Li*.Critical Reviews in Environmental Science and Technology.2026,56(16):861-885

28.A product-determining structural loop in a novel PL40 ulvan lyase controls oligosaccharide profile.Chengcheng Jiang, Chenghao Hu, Jianhua Hao*, Xiangzhao Mao*.International Journal of Biological Macromolecules.2026,377,153710:1-16

29.Structure-guided engineering of P450BM3 as a cofactor-free peroxygenase for efficient biotransformation of cresol pollutants.Yadan Yang, Xiangmin Meng, Di Yan, Ridong Yang, Feng Liu, Jiakun Xu*.Bioresource Technology.2026,443,133834:1-9

30.Development of H2O2‑Driven Peroxygenase-Type P450BM3 for Efficient Substrate Removal of Lignin-Derived Phenolics.Feng Liu,Di Yan,Lingsen Kong,Fang Wang,Qianqian Wang,Jiakun Xu *.Journal of Agricultural and Food Chemistry.2026.74 (21): 16475–16486

31.Regioselective Aromatic O‑Demethylation of Lignin-Derived Monomeric Aromatic Ethers with an Engineered P450BM3 Peroxygenase System.Sijia Zhao,Di Yan,Osami Shoji,Fang Wang,Feng Liu,Lingsen Kong,Jiakun Xu*.Journal of Agricultural and Food Chemistry.2026.56 (1): 560-568

32.Phenoxazinone synthase-like activity of rationally designed heme enzymes based on cytochrome P450BM3.Jinghan Wang, Meichun Wu, Sijia Zhao, Fang Wang, Dong Li, Lijuan Sun, Yingwu Lin*, Jiakun Xu*.Green Synthesis and Catalysis

33.Biochemical Characterization of a GH16_13 Carrageenase and Identification of Key Amino Acids Governing Product Length.Chengcheng Jiang*,  Xiangzhao Mao*.Journal of Agricultural and Food Chemistry.2026.74 (24): 18898-18908

34.Selective Aqueous Biocatalysis of D-Panthenol via Engineered Pantothenate Synthetase.Chaofan Wang, Wei Wang, Jingjing Sun, Chengcheng Jiang, Rui Wang, Jianhua Hao*.Journal of Agricultural and Food Chemistry.2026,74,13,11015–11028