主持十二五国家863子课题“海洋动物配子和胚胎冷冻保存及种质库信息化管理技术研究”,国家自然科学基金面上项目“基于转录组和microRNA 信息的牙鲆白化发生机制研究”,国家自然科学基金青年基金项目“大菱鲆Grim19基因在细胞凋亡及病原感染防御中的作用研究”,中国水产科学研究院基本科研业务费项目“半滑舌鳎雌性特异基因的表达及功能分析”等。作为科研骨干参加公益性行业科研专项、国家自然科学基金重点项目、国家十一五、十二五863项目等10余项。 1)建立并分析了多种鲆鲽鱼类细胞系,如大菱鲆肾细胞系、漠斑牙鲆肾细胞系、半滑舌鳎精巢细胞系、半滑舌鳎头肾细胞系、半滑舌鳎卵巢细胞系等,揭示这些细胞系在研究鱼类病原学和基因功能方面具有重要的应用价值,其中大菱鲆肾脏细胞系已作为一个极为重要的实验平台,被国家海洋局第一海洋研究所、上海海洋大学等多家科研单位成功应用于RNA干扰、亚细胞定位、蛋白生物活性、基因互作等多项研究中。 2)针对大菱鲆免疫抗病相关基因GRIM-19,STAT2,STAT3、HSC70、Hepcidin等,开展了免疫应答、亚细胞定位、细胞凋亡等研究,揭示了它们在抵抗鳗弧菌和淋巴囊肿病毒感染方面的重要作用,初步确认了GRIM-19和STAT3在大菱鲆细胞系中的共定位关系,鉴定了STAT2的核输出信号,这些基因的鉴定与功能分析为鲆鲽鱼类抗病分子育种策略的制定以及抗病功能基因产品的研制提供了基础资料。 3)阐明了半滑舌鳎雌性特异候选基因CSW3,CSW4等的时空表达模式,分析了不同结构域的亚细胞定位信息,鉴定了重组表达蛋白的生物学活性,构建了TALEN敲除载体并在模式鱼青鳉中进行了敲除效率的验证。 4)开展了牙鲆白化相关的转录组及miRNA高通量测序分析,初步筛选获得差异表达基因235个,差异表达miRNA46个,针对这些相关基因/miRNA进行了GO及KEGG通路富集分析,为牙鲆白化分子机制的阐示提供了丰富的分子基础。 目前已在Dev Comp Immunol、Fish Shellfish Immunol、J Fish Dis等刊物上发表论文20多篇,其中第一作者SCI文章5篇,作为第一发明人获授权专利2项,参编专著1部。
Dr Wang conducted one National Science Foundation for Young Scholars of China, one National Science Foundation of China, one 863 sub-project, and one Special Scientific Research Funds for Central Non-profit Institutes, Chinese Academy of Fishery Sciences. Also, Dr Wang participated in several projects including 973, 863, the State Key Program of National Natural Science of China, Special Fund for Agro-scientific Research in the Public Interest. 1) Dr Wang established and identified several flatfish cell lines, such as turbot kidney cell line, Southern flounder cell line, half smooth tongue sole testis cell line, and half smooth tongue sole head kidney cell line. Their application values in the fish pathogenesis and gene function were also analyzed. Especially, turbot kidney cell line has been used for the study of RNA interference, sub-cellular location, protein activity, gene interaction and so on. 2) The turbot immune related genes GRIM-19, STAT2, STAT3, HSC70 and Hepcidin were cloned and analyzed, and their possible roles against pathogen affection were revealed. The identification and functional analysis of these genes provide comprehensive basis for molecular disease-resistant breeding in aquaculture. 3) The female specific genes CSW3 and CSW4 of Half smooth tongue sole were studied by expression pattern analysis, recombinant proteins identification and TALEN analysis. 4) The transcriptome and miRNA expression pattern between albinism and normal Japanese flounder are analyzed by high-throughput sequencing. There are 235 differentially expressed genes and 46 differentially expressed miRNAs identified. Further GO and KEGG pathway enrichment were also conducted. These studies provide abundant molecular basis for exploring the mechanism of flatfish albinism. Dr Wang has published over 20 papers in Journals such as Dev Comp Immunol, Fish Shellfish Immunol, J Fish Dis and so on, of which 5 SCI papers were published as the first author. As well, as the first executers, Dr Wang also obtained two authorized patents. |